ARG42981
anti-ALKBH4 antibody
anti-ALKBH4 antibody for Western blot,Immunoprecipitation and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ALKBH4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ALKBH4 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human ALKBH4. |
| Conjugation | Un-conjugated |
| Alternate Names | ABH4; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4; Alkylated DNA repair protein alkB homolog 4; EC 1.14.11.- |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | K562 | ||||||
| Observed Size | ~ 34 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 50 nM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NXW9 Human Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4 |
|---|---|
| Gene Symbol | ALKBH4 |
| Gene Full Name | alkB homolog 4, lysine demthylase |
| Function | Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes (PubMed:23673617). Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (PubMed:23673617). In addition to proteins, also demethylates DNA: specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Midbody. Note=Associates with the contractile ring and midbody. [UniProt] |
| Calculated MW | 34 kDa |
