ARG42981

anti-ALKBH4 antibody

anti-ALKBH4 antibody for Western blot,Immunoprecipitation and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes ALKBH4
Tested Reactivity Hu
Tested Application IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ALKBH4
Antigen Species Human
Immunogen Recombinant protein of Human ALKBH4.
Conjugation Un-conjugated
Alternate Names ABH4; Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4; Alkylated DNA repair protein alkB homolog 4; EC 1.14.11.-

Application Instructions

Application Suggestion
Tested Application Dilution
IP1:20
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562
Observed Size ~ 34 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 54784 Human ALKBH4

Swiss-port # Q9NXW9 Human Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4

Gene Symbol ALKBH4
Gene Full Name alkB homolog 4, lysine demthylase
Function Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes (PubMed:23673617). Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (PubMed:23673617). In addition to proteins, also demethylates DNA: specifically demethylates DNA methylated on the 6th position of adenine (N(6)-methyladenosine) DNA, thereby regulating Polycomb silencing (By similarity). [UniProt]
Cellular Localization Cytoplasm. Nucleus. Midbody. Note=Associates with the contractile ring and midbody. [UniProt]
Calculated MW 34 kDa