ARG58849

anti-ALT / Alanine Transaminase antibody

anti-ALT / Alanine Transaminase antibody for Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ALT / Alanine Transaminase
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ALT / Alanine Transaminase
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-190 of Human ALT / Alanine Transaminase (NP_005300.1).
Conjugation Un-conjugated
Alternate Names Glutamate pyruvate transaminase 1; AAT1; GPT1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; Alanine aminotransferase 1; ALT1; EC 2.6.1.2

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:200 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat liver
Observed Size 50 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 76282 Mouse GPT

GeneID: 81670 Rat GPT

Swiss-port # P25409 Rat Alanine aminotransferase 1

Swiss-port # Q8QZR5 Mouse Alanine aminotransferase 1

Gene Symbol GPT
Gene Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Background This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
Function Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity). [UniProt]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 55 kDa