ARG63971

anti-AMPH / Amphiphysin antibody

anti-AMPH / Amphiphysin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Immune System antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes AMPH / Amphiphysin
Tested Reactivity Hu, Ms
Predict Reactivity Dog, Rat
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name AMPH / Amphiphysin
Antigen Species Human
Immunogen C-KRKDESRISKAE
Conjugation Un-conjugated
Alternate Names Amphiphysin; AMPH1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 4 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 218038 Mouse AMPH

GeneID: 273 Human AMPH

Swiss-port # P49418 Human Amphiphysin

Swiss-port # Q7TQF7 Mouse Amphiphysin

Background This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]
Research Area Immune System antibody; Neuroscience antibody
Calculated MW 76 kDa