ARG24152
anti-ANK1 / Ankyrin R antibody [S388A-10]
anti-ANK1 / Ankyrin R antibody [S388A-10] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [S388A-10] recognizes ANK1 / Ankyrin R |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S388A-10 |
| Isotype | IgG2b |
| Target Name | ANK1 / Ankyrin R |
| Antigen Species | Human |
| Immunogen | Fusion protein of Human ANK1 / Ankyrin R |
| Conjugation | Un-conjugated |
| Protein Full Name | Ankyrin-1 |
| Alternate Names | ANK1; Ankyrin 1; SPH1; ANK; Ankyrin 1, Erythrocytic ; Erythrocyte Ankyrin; Ankyrin-1; Ankyrin-R; ANK-1; SPH2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANK1 |
| Gene Full Name | Ankyrin 1 |
| Background | Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. |
| Function | Component of the ankyrin-1 complex, a multiprotein complex involved in the stability and shape of the erythrocyte membrane. |
| Cellular Localization | Cytoplasm, Cytoskeleton, Membrane, Sarcoplasmic reticulum |
| Calculated MW | 206 kDa |
| PTM | Hydroxylation, Lipoprotein, Phosphoprotein |
