ARG41196

anti-AP1M2 antibody

anti-AP1M2 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes AP1M2
Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AP1M2
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 164-423 of Human AP1M2 (NP_005489.2).
Conjugation Un-conjugated
Alternate Names HSMU1B; Golgi adaptor HA1/AP1 adaptin mu-2 subunit; MU-1B; Mu-adaptin 2; AP-mu chain family member mu1B; mu2; Adaptor-related protein complex 1 subunit mu-2; Clathrin assembly protein complex 1 mu-2 medium chain 2; MU1B; Mu1B-adaptin; AP-1 complex subunit mu-2; AP1-mu2; Adaptor protein complex AP-1 subunit mu-2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse kidney
Observed Size 48 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10053 Human AP1M2

GeneID: 11768 Mouse AP1M2

Swiss-port # Q9WVP1 Mouse AP-1 complex subunit mu-2

Swiss-port # Q9Y6Q5 Human AP-1 complex subunit mu-2

Gene Symbol AP1M2
Gene Full Name adaptor-related protein complex 1, mu 2 subunit
Background This gene encodes a subunit of the heterotetrameric adaptor-related protein comlex 1 (AP-1), which belongs to the adaptor complexes medium subunits family. This protein is capable of interacting with tyrosine-based sorting signals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Function Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. [UniProt]
Cellular Localization Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. [UniProt]
Calculated MW 48 kDa
PTM Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals. [UniProt]