ARG59798

anti-AP3B1 / HPS2 antibody

anti-AP3B1 / HPS2 antibody for Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes AP3B1 / HPS2
Tested Reactivity Hu, Ms
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AP3B1 / HPS2
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 895-1094 of Human AP3B1 (NP_003655.3).
Conjugation Un-conjugated
Alternate Names Adaptor-related protein complex 3 subunit beta-1; AP-3 complex subunit beta-1; HPS; ADTB3A; Clathrin assembly protein complex 3 beta-1 large chain; Adaptor protein complex AP-3 subunit beta-1; ADTB3; HPS2; PE; Beta-3A-adaptin

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse liver and SW620
Observed Size 132 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11774 Mouse AP3B1

GeneID: 8546 Human AP3B1

Swiss-port # O00203 Human AP-3 complex subunit beta-1

Swiss-port # Q9Z1T1 Mouse AP-3 complex subunit beta-1

Gene Symbol AP3B1
Gene Full Name adaptor-related protein complex 3, beta 1 subunit
Background This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. The encoded protein is part of the heterotetrameric AP-3 protein complex which interacts with the scaffolding protein clathrin. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2012]
Function Subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 (AP-3) that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. [UniProt]
Cellular Localization Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. [UniProt]
Calculated MW 121 kDa
PTM Phosphorylated on serine residues. [UniProt]