ARG64035

anti-APBB1 / FE65 antibody

anti-APBB1 / FE65 antibody for Western blot and Mouse

Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes APBB1 / FE65
Tested Reactivity Ms
Predict Reactivity Hu
Tested Application WB
Specificity This antibody is expected to recognise both reported isoforms (NP_001155.1 and NP_663722.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name APBB1 / FE65
Antigen Species Human
Immunogen C-GSLKPKRLGAHTP
Conjugation Un-conjugated
Alternate Names FE65; Amyloid beta A4 precursor protein-binding family B member 1; Protein Fe65; RIR; MGC:9072

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11785 Mouse APBB1

Swiss-port # Q9QXJ1 Mouse Amyloid beta A4 precursor protein-binding family B member 1

Background The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Mar 2012]
Research Area Neuroscience antibody; Signaling Transduction antibody
Calculated MW 77 kDa
PTM Phosphorylation at Ser-610 by SGK1 promotes its localization to the nucleus (By similarity). Phosphorylated following nuclear translocation. Phosphorylation at Tyr-547 by ABL1 enhances transcriptional activation activity and reduces the affinity for RASD1/DEXRAS1.