ARG63219

anti-APE1 antibody

anti-APE1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Gene Regulation antibody
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Overview

Product Description

Goat Polyclonal antibody recognizes APE1

Tested Reactivity Hu
Predict Reactivity Cow, Dog, Pig
Tested Application IHC-P, WB
Specificity Reported variants represent identical protein (NP_001632.2; NP_542379.1; NP_542380.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name APE1
Antigen Species Human
Immunogen PKRGKKGAVAEDGD-C
Conjugation Un-conjugated
Alternate Names APE1; APX; AP endonuclease 1; HAP1; EC 4.2.99.18; Apurinic-apyrimidinic endonuclease 1; APEX; apurinic or apyrimidinic site; EC 3.1.-.-; REF1; Redox factor-1; APEX nuclease; REF-1; APE-1; DNA-; APEN; APE

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P4 - 6 µg/ml
WB0.1 - 0.3 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 328 Human APEX1

Swiss-port # P27695 Human DNA-(apurinic or apyrimidinic site) lyase

Gene Symbol APEX1
Gene Full Name APEX nuclease (multifunctional DNA repair enzyme) 1
Background Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 36 kDa
PTM Phosphorylated. Phosphorylation by kinase PKC or casein kinase CK2 results in enhanced redox activity that stimulates binding of the FOS/JUN AP-1 complex to its cognate binding site. AP-endodeoxyribonuclease activity is not affected by CK2-mediated phosphorylation. Phosphorylation of Thr-233 by CDK5 reduces AP-endodeoxyribonuclease activity resulting in accumulation of DNA damage and contributing to neuronal death.
Acetylated on Lys-6 and Lys-7. Acetylation is increased by the transcriptional coactivator EP300 acetyltransferase, genotoxic agents like H(2)O(2) and methyl methanesulfonate (MMS). Acetylation increases its binding affinity to the negative calcium response element (nCaRE) DNA promoter. The acetylated form induces a stronger binding of YBX1 to the Y-box sequence in the MDR1 promoter than the unacetylated form. Deacetylated on lysines. Lys-6 and Lys-7 are deacetylated by SIRT1.
Cleaved at Lys-31 by granzyme A to create the mitochondrial form; leading in reduction of binding to DNA, AP endodeoxynuclease activity, redox activation of transcription factors and to enhanced cell death. Cleaved by granzyme K; leading to intracellular ROS accumulation and enhanced cell death after oxidative stress.
Cys-65 and Cys-93 are nitrosylated in response to nitric oxide (NO) and lead to the exposure of the nuclear export signal (NES).
Ubiquitinated by MDM2; leading to translocation to the cytoplasm and proteasomal degradation.

Customer's Feedback

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Review for anti-APE1 antibody

Application:WB

Sample:293T and HeLa cells

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1 μg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC