ARG40373

anti-APLP1 antibody

anti-APLP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes APLP1
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Bov
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name APLP1
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 101-120 of Human APLP1. (ELQIARVEQATQAIPMERWC)
Conjugation Un-conjugated
Alternate Names APLP; Amyloid-like protein 1; APLP-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: By heat mediation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 72 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Thimerosal and 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11803 Mouse APLP1

GeneID: 333 Human APLP1

Swiss-port # P51693 Human Amyloid-like protein 1

Swiss-port # Q03157 Mouse Amyloid-like protein 1

Gene Symbol APLP1
Gene Full Name amyloid beta (A4) precursor-like protein 1
Background This gene encodes a member of the highly conserved amyloid precursor protein gene family. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Function May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding (By similarity). Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.

The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. C30: Cytoplasm. Note=C-terminally processed in the Golgi complex. [UniProt]
Calculated MW 72 kDa
PTM Proteolytically cleaved by caspases during neuronal apoptosis. Cleaved, in vitro, at Asp-620 by caspase-3 (By similarity).

N- and O-glycosylated. O-glycosylation with core 1 or possibly core 8 glycans. Glycosylation on Ser-227 is the preferred site to Thr-228. [UniProt]