ARG64741

anti-APOBEC3G antibody

anti-APOBEC3G antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody; Immune System antibody; Microbiology and Infectious Disease antibody

Overview

Product Description Goat Polyclonal antibody recognizes APOBEC3G
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name APOBEC3G
Antigen Species Human
Immunogen C-DEHSQDLSGRLR
Conjugation Un-conjugated
Alternate Names ARP-9; APOBEC-related cytidine deaminase; Deoxycytidine deaminase; APOBEC-related protein; dJ494G10.1; DNA dC->dU-editing enzyme APOBEC-3G; CEM-15; CEM15; ARCD; ARP9; APOBEC-related protein 9; EC 3.5.4.-; A3G; bK150C2.7; MDS019

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2.5 µg/ml
WB0.5 - 1.5 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 60489 Human APOBEC3G

Swiss-port # Q9HC16 Human DNA dC->dU-editing enzyme APOBEC-3G

Background This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity. [provided by RefSeq, Jul 2008]
Research Area Gene Regulation antibody; Immune System antibody; Microbiology and Infectious Disease antibody
Calculated MW 46 kDa
PTM Ubiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway.
Phosphorylation at Thr-32 reduces its binding to HIV-1 VIF and subsequent ubiquitination and degradation thus promoting its antiviral activity.