ARG56849
anti-ARFBP1 antibody
anti-ARFBP1 antibody for Western blot,ICC/IF and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ARFBP1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ARFBP1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (19 aa) within aa. 4250-4300 of Human ARF-BP1. |
| Conjugation | Un-conjugated |
| Alternate Names | HECT, UBA and WWE domain-containing protein 1; HectH9; EC 6.3.2.-; HSPC272; ARF-binding protein 1; Mule; Homologous to E6AP carboxyl terminus homologous protein 9; E3 ubiquitin-protein ligase HUWE1; HECTH9; ARF-BP1; URE-binding protein 1; Ib772; Mcl-1 ubiquitin ligase E3; LASU1; UREB1; Large structure of UREB1; MULE; Upstream regulatory element-binding protein 1; URE-B1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Daudi cell lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HUWE1 |
| Gene Full Name | HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase |
| Background | This gene encodes a protein containing a C-terminal HECT (E6AP type E3 ubiquitin protein ligase) domain that functions as an E3 ubiquitin ligase. The encoded protein is required for the ubiquitination and subsequent degradation of the anti-apoptotic protein Mcl1 (myeloid cell leukemia sequence 1 (BCL2-related)). This protein also ubiquitinates the p53 tumor suppressor, core histones, and DNA polymerase beta. Mutations in this gene are associated with Turner type X-linked syndromic mental retardation. [provided by RefSeq, Aug 2013] |
| Function | E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. Mediates polyubiquitination of isoform 2 of PA2G4. [UniProt] |
| Calculated MW | 482 kDa |
| PTM | Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation. |
