ARG42367
anti-ARHGEF4 antibody [ARHGEF-08]
anti-ARHGEF4 antibody [ARHGEF-08] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [ARHGEF-08] recognizes ARHGEF4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Specificity | The mouse monoclonal antibody ARHGEF-08 recognizes human intracellular protein ARHGEF4 / ASEF1, approx. 80 kDa guanine nucleotide exchange factor specific for Rac1 and Cdc42. The epitope is located in the rough at the region of SH3 domain. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ARHGEF-08 |
| Isotype | IgG1 |
| Target Name | ARHGEF4 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment corresponding to aa. 143-271 of Human ARHGEF4. |
| Conjugation | Un-conjugated |
| Alternate Names | STM6; Rho guanine nucleotide exchange factor 4; Asef; GEF4; APC-stimulated guanine nucleotide exchange factor 1; Asef1; ASEF; ASEF1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NR80 Human Rho guanine nucleotide exchange factor 4 |
|---|---|
| Gene Symbol | ARHGEF4 |
| Gene Full Name | Rho guanine nucleotide exchange factor (GEF) 4 |
| Background | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The protein encoded by this gene may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jun 2013] |
| Function | Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. [UniProt] |
| Cellular Localization | Isoform 3: Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated with membrane ruffles. [UniProt] |
| Calculated MW | 79 kDa |
