ARG42096

anti-ARPC1A antibody

anti-ARPC1A antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Goat Polyclonal antibody recognizes ARPC1A
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow, Pig
Tested Application IHC-P, WB
Specificity This antibody is expected to recognize both reported isoforms (NP_006400.2; NP_001177925.1). No cross-reactiviry expected with ARPC1B.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ARPC1A
Antigen Species Human
Immunogen Synthetic peptide around the C-terminal region of Human ARPC1A. (C-DFKTLESSIQGLR) (NP_006400.2; NP_001177925.1)
Conjugation Un-conjugated
Alternate Names SOP2L; Actin-related protein 2/3 complex subunit 1A; SOP2Hs; HEL-68; Arc40; SOP2-like protein

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 42 kDa (Human)

Properties

Form Liquid
Purification Ammonium sulphate precipitation followed by affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10552 Human ARPC1A

GeneID: 56443 Mouse ARPC1A

Swiss-port # Q92747 Human Actin-related protein 2/3 complex subunit 1A

Swiss-port # Q9R0Q6 Mouse Actin-related protein 2/3 complex subunit 1A

Gene Symbol ARPC1A
Gene Full Name actin related protein 2/3 complex, subunit 1A, 41kDa
Background This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
Function Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton. [UniProt]
Calculated MW 42 kDa (Human)