ARG63465

anti-ARPC2 antibody

anti-ARPC2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ARPC2
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog
Tested Application ICC/IF, IHC-P, WB
Specificity The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ARPC2
Antigen Species Human
Immunogen C-EMKTITGKTFSSR
Conjugation Un-conjugated
Alternate Names Arp2/3 complex 34 kDa subunit; PNAS-139; p34-ARC; Actin-related protein 2/3 complex subunit 2; ARC34; p34-Arc; PRO2446

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF10 µg/ml
IHC-P3 - 5 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10109 Human ARPC2

Swiss-port # O15144 Human Actin-related protein 2/3 complex subunit 2

Background This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 34 kDa