ARG41366

anti-ARPC5 / p16 ARC antibody

anti-ARPC5 / p16 ARC antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ARPC5 / p16 ARC
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ARPC5 / p16 ARC
Antigen Species Human
Immunogen Synthetic peptide derived from Human ARPC5 / p16 ARC.
Conjugation Un-conjugated
Alternate Names p16-Arc; p16-ARC; Actin-related protein 2/3 complex subunit 5; ARC16; Arp2/3 complex 16 kDa subunit; dJ127C7.3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:100
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 17 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10092 Human ARPC5

GeneID: 360854 Rat ARPC5

GeneID: 67771 Mouse ARPC5

Gene Symbol ARPC5
Gene Full Name actin related protein 2/3 complex, subunit 5, 16kDa
Background This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p16 subunit, has yet to be determined. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
Function Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton. Cell projection. [UniProt]
Calculated MW 16 kDa
PTM Polyubiquitinated by RNF128 with 'Lys-63'-linked chains, leading to proteasomal degradation. [UniProt]