ARG10956

anti-ASAP1 antibody [7B12]

anti-ASAP1 antibody [7B12] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Rat

publication_link Publication1

Overview

Product Description Mouse Monoclonal antibody [7B12] recognizes ASAP1
Tested Reactivity Hu, Rat
Tested Application ELISA, IHC-Fr, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone 7B12
Isotype IgG2a
Target Name ASAP1
Antigen Species Human
Immunogen Recombinant Protein of Human ASAP1 with N-terminal GST tag.
Conjugation Un-conjugated
Alternate Names PAP; Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1; 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein; ARF GTPase-activating protein 1; CENTB4; DDEF1; PIP2-dependent ARF1 GAP; PAG2; ZG14P; Differentiation-enhancing factor 1; AMAP1; ADP-ribosylation factor-directed GTPase-activating protein 1; DEF-1; Development and differentiation-enhancing factor 1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
IHC-Fr5 µg/ml
IHC-P5 µg/ml
IP10 µg/ml
WB1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human: HT29 and MDA-MB-231. Rat: ASAP1 is strongly expressed in ASML and weakly expressed in 1AS pancreatic carcinoma cells.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 314961 Rat ASAP1

GeneID: 50807 Human ASAP1

Swiss-port # Q1AAU6 Rat Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Swiss-port # Q9ULH1 Human Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1

Gene Symbol ASAP1
Gene Full Name ArfGAP with SH3 domain, ankyrin repeat and PH domain 1
Background This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
Function Possesses phosphatidylinositol 4,5-bisphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity). Plays a role in ciliogenesis. [UniProt]
Calculated MW 125 kDa
PTM Phosphorylated on tyrosine residues by SRC. [UniProt]

Specific References

Probing the Expression of ASAP1 and FAK in nonsmall cell lung cancer and their clinicopathological significance

IHC-P / Human

Qixian Zheng et al.
,  (2023)

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