ARG43170
anti-ASH2L antibody
anti-ASH2L antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ASH2L |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ASH2L |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human ASH2L. |
| Conjugation | Un-conjugated |
| Alternate Names | ASH2-like protein; ASH2L1; ASH2L2; Set1/Ash2 histone methyltransferase complex subunit ASH2; Bre2; ASH2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | K562 | ||||||||
| Observed Size | 69, 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q91X20 Mouse Set1/Ash2 histone methyltransferase complex subunit ASH2 Swiss-port # Q9UBL3 Human Set1/Ash2 histone methyltransferase complex subunit ASH2 |
|---|---|
| Gene Symbol | ASH2L |
| Gene Full Name | ash2 (absent, small, or homeotic)-like (Drosophila) |
| Function | Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 69 kDa |
| PTM | Both monomethylated and dimethylated on arginine residues in the C-terminus. Arg-296 is the major site. Methylation is not required for nuclear localization, nor for MLL complex integrity or maintenance of global histone H3K4me3 levels. [UniProt] |
