ARG22245
anti-ASIC1 antibody [S271-44]
anti-ASIC1 antibody [S271-44] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [S271-44] recognizes ASIC1 |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Detects ~60kDa. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S271-44 |
| Isotype | IgG1 |
| Target Name | ASIC1 |
| Antigen Species | Mouse |
| Immunogen | Fusion protein amino acids 460-526 (Cytoplasmic C-terminus) of Mouse ASIC1 |
| Conjugation | Un-conjugated |
| Alternate Names | ACCN2; ASIC; Amiloride-sensitive cation channel 2, neuronal; ASIC1; BNaC2; Acid-sensing ion channel 1; Brain sodium channel 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Asic1 |
| Gene Full Name | acid-sensing (proton-gated) ion channel 1 |
| Background | This gene encodes a member of the acid-sensing ion channel (ASIC) family of proteins, which are part of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. Members of the ASIC family are sensitive to amiloride and function in neurotransmission. The encoded proteins function in learning, pain transduction, touch sensation, and development of memory and fear. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2012] |
| Function | Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear. Isoform 1 does not display proton-gated cation channel activity. [UniProt] |
| Cellular Localization | Cell membrane |
| Calculated MW | 60 kDa |
| PTM | Phosphorylation by PKA regulates interaction with PRKCABP and subcellular location. Phosphorylation by PKC may regulate the channel. |
