ARG44701
anti-ASK1 antibody
anti-ASK1 antibody for Immunoprecipitation and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes ASK1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | ASK1 |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | ASK-1; MEK kinase 5; ASK1; MAPK/ERK kinase kinase 5; Mitogen-activated protein kinase kinase kinase 5; MEKK5; Apoptosis signal-regulating kinase 1; MEKK 5; EC 2.7.11.25; MAPKKK5; M3K5 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purification |
| Buffer | PBS with 0.09% sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99683 Human Mitogen-activated protein kinase kinase kinase 5 |
|---|---|
| Gene Symbol | MAP3K5 |
| Gene Full Name | mitogen-activated protein kinase kinase kinase 5 |
| Background | Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death. |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1). [UniProt] |
| Cellular Localization | Cytoplasm, Endoplasmic reticulum |
| PTM | N-glycosylation enhances cell surface expression and lengthens receptor half-life by preventing degradation in the ER. |
