ARG57134

anti-ASNA1 antibody [2A1]

anti-ASNA1 antibody [2A1] for Western blot,Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody [2A1] recognizes ASNA1
Tested Reactivity Hu
Tested Application FACS, WB
Host Mouse
Clonality Monoclonal
Clone 2A1
Isotype IgG2b, kappa
Target Name ASNA1
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-348 of Human ASNA1
Conjugation Un-conjugated
Alternate Names EC 3.6.-.-; GET3; ARSA-I; Arsenite-stimulated ATPase; ATPase ASNA1; hARSA-I; hASNA-I; Transmembrane domain recognition complex 40 kDa ATPase subunit; ARSA1; TRC40; ASNA-I; Arsenical pump-driving ATPase

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 439 Human ASNA1

Swiss-port # O43681 Human ATPase ASNA1

Gene Symbol ASNA1
Gene Full Name arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Background This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion. [provided by RefSeq, Oct 2011]
Function ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity). May be involved in insulin signaling. [UniProt]
Calculated MW 39 kDa