ARG55294

anti-ASNA1 antibody

anti-ASNA1 antibody for Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ASNA1
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ASNA1
Antigen Species Human
Immunogen Recombinant protein of Human ASNA1 (NP_004308.2)
Conjugation Un-conjugated
Alternate Names EC 3.6.-.-; GET3; ARSA-I; Arsenite-stimulated ATPase; ATPase ASNA1; hARSA-I; hASNA-I; Transmembrane domain recognition complex 40 kDa ATPase subunit; ARSA1; TRC40; ASNA-I; Arsenical pump-driving ATPase

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:200 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat brain, Mouse kidney and MCF7
Observed Size ~ 40 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 439 Human ASNA1

GeneID: 56495 Mouse ASNA1

Swiss-port # O43681 Human ATPase ASNA1

Swiss-port # O54984 Mouse ATPase Asna1

Gene Symbol ASNA1
Gene Full Name arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Background This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion. [provided by RefSeq, Oct 2011]
Function ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity). May be involved in insulin signaling. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 39 kDa