ARG63742

anti-ASPN / Asporin antibody

anti-ASPN / Asporin antibody for ICC/IF,Western blot and Human,Mouse,Rat

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes ASPN / Asporin
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ASPN / Asporin
Antigen Species Human
Immunogen C-IHENKVKKIQKDT
Conjugation Un-conjugated
Alternate Names Periodontal ligament-associated protein 1; OS3; SLRR1C; Asporin; PLAP-1; PLAP1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF10 µg/ml
WB0.1 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by antigen affinity chromatography.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 54829 Human ASPN

GeneID: 66695 Mouse ASPN

Swiss-port # Q99MQ4 Mouse Asporin

Swiss-port # Q9BXN1 Human Asporin

Background This gene encodes a cartilage extracellular protein that is member of the small leucine-rich proteoglycan family. The encoded protein may regulate chondrogenesis by inhibiting transforming growth factor-beta 1-induced gene expression in cartilage. This protein also binds collagen and calcium and may induce collagen mineralization. Polymorphisms in the aspartic acid repeat region of this gene are associated with a susceptibility to osteoarthritis. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jul 2010]
Research Area Signaling Transduction antibody
Calculated MW 43 kDa
PTM There is no serine/glycine dipeptide sequence expected for the attachment of O-linked glycosaminoglycans and this is probably not a proteoglycan. The O-linked polysaccharide on 54-Ser is probably the mucin type linked to GalNAc.
The N-linked glycan at Asn-282 is composed of variable structures of GlcNAc, mannose, fucose, HexNAc and hexose.