ARG54919

anti-ATG101 antibody

anti-ATG101 antibody for ELISA,Immunohistochemistry,Western blot,ICC/IF and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Cell Death antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ATG101
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATG101
Antigen Species Human
Immunogen Synthetic peptide (16 aa) within aa. 80-130 of Human ATG101.
Conjugation Un-conjugated
Alternate Names Autophagy-related protein 101; C12orf44

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF20 μg/ml
IHC5 μg/ml
WB1 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human Liver Tissue Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 300240 Rat ATG101

GeneID: 60673 Human ATG101

GeneID: 68118 Mouse ATG101

Gene Symbol ATG101
Gene Full Name autophagy related 101
Background ATG101 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG101 is a recently discovered protein that stabilizes ATG13, another autophagy protein that forms a complex with the mammalian homologs of ATG1, ULK1 and ULK2, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. ATG101 also interacts with ULK1 and is essential for autophagy.
Function Autophagy factor required for autophagosome formation. Stabilizes ATG13, protecting it from proteasomal degradation. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody
Calculated MW 25 kDa