ARG40594

anti-ATG13 antibody

anti-ATG13 antibody for Western blot,Flow cytometry,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ATG13
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATG13
Antigen Species Human
Immunogen Recombinant protein of Human ATG13.
Conjugation Un-conjugated
Alternate Names KIAA0652; Autophagy-related protein 13; PARATARG8

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:20
IP1:20
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 51897 Mouse ATG13

GeneID: 9776 Human ATG13

Swiss-port # O75143 Human Autophagy-related protein 13

Swiss-port # Q91YI1 Mouse Autophagy-related protein 13

Gene Symbol ATG13
Gene Full Name autophagy related 13
Function Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation. [UniProt]
Cellular Localization Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation. [UniProt]
Calculated MW 57 kDa
PTM Phosphorylated by ULK1, ULK2 and mTOR. Phosphorylation status depends on nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. ULK1-mediated phosphorylation of ATG13 at Ser-355 is required for efficient clearance of depolarized mitochondria. [UniProt]