ARG64552

anti-ATG16L1 antibody

anti-ATG16L1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes ATG16L1
Tested Reactivity Hu, Ms
Predict Reactivity Dog, Rat
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms (NP_110430.5 and NP_060444.3).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ATG16L1
Antigen Species Human
Immunogen C-KVEKVLSKQHSSSIN
Conjugation Un-conjugated
Alternate Names IBD10; APG16L; ATG16L; WDR30; Autophagy-related protein 16-1; APG16-like 1; ATG16A

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P3 - 5 µg/ml
WB0.01 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 55054 Human ATG16L1

GeneID: 77040 Mouse ATG16L1

Swiss-port # Q676U5 Human Autophagy-related protein 16-1

Swiss-port # Q8C0J2 Mouse Autophagy-related protein 16-1

Background The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 68 kDa
PTM Proteolytic cleavage by activated CASP3 leads to degradation and may regulate autophagy upon cellular stress and apoptotic stimuli.
Phosphorylation at Ser-139 promotes association with the ATG12-ATG5 conjugate to form the ATG12-ATG5-ATG16L1 complex.