ARG54904

anti-ATG4A antibody

anti-ATG4A antibody for Immunoprecipitation,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
publication_link Publication1

Overview

Product Description

Rabbit Polyclonal antibody recognizes ATG4A

Tested Reactivity Hu, Ms
Tested Application IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATG4A
Antigen Species Human
Immunogen Recombinant protein of Human ATG4A (NP_443168.2)
Conjugation Un-conjugated
Alternate Names Cysteine protease ATG4A; Autophagin-2; AUT-like 2 cysteine endopeptidase; APG4A; Autophagy-related cysteine endopeptidase 2; EC 3.4.22.-; AUTL2; Autophagy-related protein 4 homolog A; hAPG4A

Application Instructions

Application Suggestion
Tested Application Dilution
IP1:20 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control BT474

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 115201 Human ATG4A

GeneID: 666468 Mouse ATG4A

Swiss-port # Q8C9S8 Mouse Cysteine protease ATG4A

Swiss-port # Q8WYN0 Human Cysteine protease ATG4A

Gene Symbol ATG4A
Gene Full Name autophagy related 4A, cysteine peptidase
Background Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Function Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Calculated MW 45 kDa

Specific References

Analysis of ATG4C function in vivo

WB / Human

Isaac Tamargo-Gómez et al.
Autophagy.,  (2023)

publication_link

 

hr_line