ARG54916

anti-ATG7 antibody

anti-ATG7 antibody for ELISA,Western blot,ICC/IF and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ATG7
Tested Reactivity Hu, Ms
Tested Application ELISA, ICC/IF, WB
Specificity At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATG7
Antigen Species Human
Immunogen Synthetic peptide (17 aa) within aa. 590-640 of Human APG7.
Conjugation Un-conjugated
Alternate Names Ubiquitin-like modifier-activating enzyme ATG7; hAGP7; Autophagy-related protein 7; GSA7; Ubiquitin-activating enzyme E1-like protein; APG7-LIKE; APG7L; APG7-like; ATG12-activating enzyme E1 ATG7

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF10 - 20 μg/ml
WB0.5 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Caco-2 Cell Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10533 Human ATG7

GeneID: 74244 Mouse ATG7

Swiss-port # O95352 Human Ubiquitin-like modifier-activating enzyme ATG7

Swiss-port # Q9D906 Mouse Ubiquitin-like modifier-activating enzyme ATG7

Gene Symbol ATG7
Gene Full Name autophagy related 7
Background APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death.
Function E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 78 kDa
PTM Acetylated by EP300.