ARG54916
anti-ATG7 antibody
anti-ATG7 antibody for ELISA,Western blot,ICC/IF and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATG7 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, ICC/IF, WB |
| Specificity | At least three isoforms of APG7 are known to exist; this antibody will detect all three isoforms. APG7 antibody is predicted not to cross-react with other ATG family proteins. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATG7 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (17 aa) within aa. 590-640 of Human APG7. |
| Conjugation | Un-conjugated |
| Alternate Names | Ubiquitin-like modifier-activating enzyme ATG7; hAGP7; Autophagy-related protein 7; GSA7; Ubiquitin-activating enzyme E1-like protein; APG7-LIKE; APG7L; APG7-like; ATG12-activating enzyme E1 ATG7 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Caco-2 Cell Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95352 Human Ubiquitin-like modifier-activating enzyme ATG7 Swiss-port # Q9D906 Mouse Ubiquitin-like modifier-activating enzyme ATG7 |
|---|---|
| Gene Symbol | ATG7 |
| Gene Full Name | autophagy related 7 |
| Background | APG7 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death. |
| Function | E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 78 kDa |
| PTM | Acetylated by EP300. |
