ARG54920

anti-ATG9A antibody

anti-ATG9A antibody for ELISA,Immunohistochemistry,Western blot,ICC/IF and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes ATG9A
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATG9A
Antigen Species Human
Immunogen Synthetic peptide (18 aa) within aa. 720-770 of Human ATG9A.
Conjugation Un-conjugated
Alternate Names APG9L1; MGD3208; APG9-like 1; mATG9; Autophagy-related protein 9A

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF20 μg/ml
IHC5 μg/ml
WB1 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse Heart Tissue Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 245860 Mouse ATG9A

GeneID: 363254 Rat ATG9A

GeneID: 79065 Human ATG9A

Gene Symbol ATG9A
Gene Full Name autophagy related 9A
Background ATG9A Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9A, a multi-spanning membrane protein localizing to the Golgi apparatus and late endosomes, has been proposed to mediate membrane transport to generate autophagosomes. ATG9A has also been implicated as a regulator of STING (stimulator of interferon genes)-mediated innate immune response.
Function Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 94 kDa