ARG58285

anti-ATP2A1 / SERCA1 ATPase (isoform b) antibody

anti-ATP2A1 / SERCA1 ATPase (isoform b) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes ATP2A1 / SERCA1 ATPase (isoform b)
Tested Reactivity Hu
Predict Reactivity Rb
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATP2A1 / SERCA1 ATPase (isoform b)
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 974-1001 of Human ATP2A1 / SERCA1 ATPase (isoform b).
Conjugation Un-conjugated
Alternate Names SERCA1; ATP2A; SR Ca; Endoplasmic reticulum class 1/2 Ca; EC 3.6.3.8; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; 2+

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HT1080

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 487 Human ATP2A1

Swiss-port # O14983 Human Sarcoplasmic/endoplasmic reticulum calcium ATPase 1

Gene Symbol ATP2A1
Gene Full Name ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
Background This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]
Function Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. [UniProt]
Cellular Localization Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt]
Calculated MW 110 kDa