ARG58316

anti-ATP2A3 / SERCA3 ATPase antibody

anti-ATP2A3 / SERCA3 ATPase antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes ATP2A3 / SERCA3 ATPase
Tested Reactivity Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name ATP2A3 / SERCA3 ATPase
Antigen Species Human
Immunogen Synthetic peptide corresponding to a sequence at the N-terminus of Human ATP2A3(1-30aa MEAAHLLPAADVLRHFSVTAEGGLSPAQVT), different from the related Mouse sequence by five amino acids, and from the related Rat sequence by six amino acids.
Conjugation Un-conjugated
Alternate Names SERCA3; SR Ca; Sarcoplasmic/endoplasmic reticulum calcium ATPase 3; EC 3.6.3.8; Calcium pump 3; 2+

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: By heat mediation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25391 Rat ATP2A3

GeneID: 53313 Mouse ATP2A3

Swiss-port # P18596 Rat Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

Swiss-port # Q64518 Mouse Sarcoplasmic/endoplasmic reticulum calcium ATPase 3

Gene Symbol ATP2A3
Gene Full Name ATPase, Ca++ transporting, ubiquitous
Background This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Function This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. [UniProt]
Cellular Localization Nucleus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein. [UniProt]
Calculated MW 114 kDa