ARG65744
anti-ATP2B1 / PMCA1 antibody
anti-ATP2B1 / PMCA1 antibody for Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes ATP2B1 / PMCA1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001001323.1; NP_001673.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATP2B1 / PMCA1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human ATP2B1. (C-KQDGAIENRNKAKAQD) |
| Conjugation | Un-conjugated |
| Alternate Names | PMCA1; PMCA1kb; EC 3.6.3.8; Plasma membrane calcium ATPase isoform 1; Plasma membrane calcium pump isoform 1; Plasma membrane calcium-transporting ATPase 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P20020 Human Plasma membrane calcium-transporting ATPase 1 |
|---|---|
| Gene Symbol | ATP2B1 |
| Gene Full Name | ATPase, Ca++ transporting, plasma membrane 1 |
| Background | The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Function | This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. [UniProt] |
| Calculated MW | 135 kDa (NP_001673.2) |
