ARG52233
anti-ATRIP phospho (Ser239) antibody
anti-ATRIP phospho (Ser239) antibody for Western blot and Mouse
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ATRIP phospho (Ser239) |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Hu, Bov, NHuPrm, Sheep |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ATRIP |
| Antigen Species | Human |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | ATR-interacting protein; ATM and Rad3-related-interacting protein |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | Specific for the ~86k ATRIP protein phosphorylated at Ser239. The antibody also recognizes a band at ~32k. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ATRIP |
| Gene Full Name | ATR interacting protein |
| Background | ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001). ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007). DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007). The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at ser239 (Venere et al, 2007). |
| Research Area | Gene Regulation antibody |
| Calculated MW | 86 kDa |
| PTM | Phosphorylated by ATR. |
