ARG52223
anti-Actin antibody [C4]
anti-Actin antibody [C4] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Bovine,C. elegans,Chicken,D. melanogaster ,Dictyostelium discoideum,Human,Mouse,Pig,Rabbit,Rat,Xenopus,Zebrafish
Cancer antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [C4] recognizes Actin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Ce, Chk, D. discoideum, Dm, Pig, Rb, Xenopus, Zfsh |
| Predict Reactivity | Mamm, Vt |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C4 |
| Isotype | IgG1 |
| Target Name | Actin |
| Antigen Species | Chicken |
| Immunogen | Preparation of chicken gizzard actin |
| Conjugation | Un-conjugated |
| Alternate Names | CFTDM; MPFD; CFTD; ASMA; NEM1; NEM2; NEM3; Alpha-actin-1; ACTA; CFTD1; Actin, alpha skeletal muscle |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~42 kDa actin protein in lysates from skeletal, cardiac, gizzard and aorta tissues. Reacts with all actin isoforms. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Ascites fluid |
| Buffer | Ascites fluid and 0.01 % Sodium azide |
| Preservative | 0.01 % Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ACTA1 |
| Gene Full Name | actin, alpha 1, skeletal muscle |
| Background | Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton. |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt] |
| Research Area | Cancer antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 42 kDa |
| PTM | Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity). Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148). |
Clone References
Deteriorated stress response in stationary-phase yeast: Sir2 and Yap1 are essential for Hsf1 activation by heat shock and oxidative stress, respectively.
WB / Saccharomyces cerevisiae
A functional MiR-124 binding-site polymorphism in IQGAP1 affects human cognitive performance.
WB / Human
myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis.
WB / Mouse
Identification of genes in toxicity pathways of trinucleotide-repeat RNA in C. elegans.
SNAP23, Syntaxin4, and vesicle-associated membrane protein 7 (VAMP7) mediate trafficking of membrane type 1-matrix metalloproteinase (MT1-MMP) during invadopodium formation and tumor cell invasion.
ICC/IF / Human
Dichloroacetate prevents restenosis in preclinical animal models of vessel injury.
Casein kinase 1 regulates Sprouty2 in FGF-ERK signaling.
Altered migratory behavior of interneurons in a model of cortical dysplasia: the influence of elevated GABAA activity.
WB / Ferret
Overexpression of ABCG1 protein attenuates arteriosclerosis and endothelial dysfunction in atherosclerotic rabbits.
IHC / Rabbit
N-glycosylation gene DPAGT1 is a target of the Wnt/beta-catenin signaling pathway.
ICC/IF / Human
Hepatocyte growth factor improves viability after H2O2-induced toxicity in bile duct epithelial cells.
WB / Dog
