ARG63666

anti-Akt 3 antibody

anti-Akt 3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Akt 3
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Cow, Dog
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2. Reported variants represent identical protein (NP_859029.1; NP_001193658.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Akt 3
Antigen Species Human
Immunogen CSPTSQIDNIGEEEM
Conjugation Un-conjugated
Alternate Names Protein kinase Akt-3; PKB-GAMMA; PKB gamma; RAC-gamma serine/threonine-protein kinase; STK-2; PRKBG; RAC-gamma; MPPH2; MPPH; Protein kinase B gamma; EC 2.7.11.1; PKBG; RAC-PK-gamma

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10000 Human AKT3

GeneID: 23797 Mouse AKT3

GeneID: 29414 Rat AKT3

Background The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 56 kDa
PTM Phosphorylation on Thr-305 and Ser-472 is required for full activity.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome.
O-GlcNAcylation at Thr-302 and Thr-309 inhibits activating phosphorylation at Thr-305 via disrupting the interaction between AKT and PDK1.