ARG53745

anti-Albumin antibody [AL-01] (Biotin)

anti-Albumin antibody [AL-01] (Biotin) for Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [AL-01] recognizes Albumin
Tested Reactivity Hu
Tested Application WB
Specificity The clone AL-01 reacts only with human serum albumin, a 65-67 kDa monomeric protein in human blood plasma; it is produced in liver.
No cross-reactivity was observed with other serum proteins.
Host Mouse
Clonality Monoclonal
Clone AL-01
Isotype IgG1
Target Name Albumin
Antigen Species Human
Immunogen Fraction of proteins containing albumin after ammonnium sulphate precipitation and DEAE-chromatography of human serum.
Conjugation Biotin
Alternate Names FDAH; PRO0883; PRO1341; Serum albumin; ANALBA; PRO0903

Application Instructions

Application Suggestion
Tested Application Dilution
WB2 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 213 Human ALB

Swiss-port # P02768 Human Serum albumin

Gene Symbol ALB
Gene Full Name albumin
Background Human serum albumin (65-67 kDa) is the most abundant protein in human blood plasma (produced in the liver). It has a serum half-life of approximately 20 days.
Function Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Developmental Biology antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 69 kDa
PTM Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylated by FAM20C in the extracellular medium.
Acetylated on Lys-223 by acetylsalicylic acid.

Clone References

Targeting the ion channel Kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-life.

ELISA / 

Edwards W et al.
J Biol Chem.,  (2014)

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Human immunodeficiency virus (HIV) antigens and RNA in HIV-seronegative women with cervical intraepithelial neoplasia.

WB / Human

Basu J et al.
AIDS Res Hum Retroviruses.,  (2009)

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The characteristics of monoclonal antibodies against human albumin.

Angelisová P et al.
Folia Biol (Praha).,  (1986)

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