ARG23504
anti-Angiotensin I antibody [13/96/Ang C9 (BGN/KA/22H)]
anti-Angiotensin I antibody [13/96/Ang C9 (BGN/KA/22H)] for ELISA and Human
Overview
| Product Description | Mouse Monoclonal antibody [13/96/Ang C9 (BGN/KA/22H)] recognizes Angiotensin I. Mouse anti Human angiotensin I antibody, clone 13/96/Ang C9 recognizes angiotensin I, a 10 amino acid peptide derived by the proteolytic processing of angiotensinogen. Angiotensin I is important in the Renin-Angiotensin-Aldosterone System (RAAS). RAAS is responsible for the regulation of blood pressure and water retention in the body. Angiotensin I is produced from a reaction between the renal enzyme Renin and angiotensinogen. It is further converted by Angiotensin Converting Enzyme (ACE) into a vasoactive form called Angiotensin II which is responsible for increasing blood pressure. Mouse anti Human angiotensin I antibody does not react with angiotensin II, III or angiotensinogen in ELISA. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 13/96/Ang C9 (BGN/KA/22H) |
| Isotype | IgG2b |
| Target Name | Angiotensin I |
| Antigen Species | Human |
| Immunogen | HMW conjugate. |
| Conjugation | Un-conjugated |
| Alternate Names | Des-Asp[1]-angiotensin II; Angiotensin III; SERPINA8; Angiotensinogen; Angiotensin 3-8; Ang IV; Ang I; Angiotensin I; Angiotensin II; Angiotensin 1-8; Angiotensin 1-10; Angiotensin IV; Ang III; Ang II; Angiotensin 2-8; ANHU; Serpin A8 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | Indirect ELISA: Using 1.0 µg of Angiotensin I coated per well, the 50% binding was observed at an antibody dilution of 1:950. Under the same conditions, the end point titre was observed at an antibody dilution of 1:10000. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AGT |
| Gene Full Name | angiotensinogen (serpin peptidase inhibitor, clade A, member 8) |
| Background | The protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease. [provided by RefSeq, Jul 2008] |
| Function | Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2: acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Angiotensin-3: stimulates aldosterone release. Angiotensin 1-7: is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. [UniProt] |
| Calculated MW | 53 kDa |
| PTM | Beta-decarboxylation of Asp-34 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin. [UniProt] |
