ARG64834

anti-Annexin A1 antibody

anti-Annexin A1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat,Pig

Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Annexin A1
Tested Reactivity Hu, Ms, Rat, Pig
Predict Reactivity Cow, Dog
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Annexin A1
Antigen Species Human
Immunogen CQAILDETKGDYEK
Conjugation Un-conjugated
Alternate Names ANX1; Calpactin II; Phospholipase A2 inhibitory protein; Chromobindin-9; LPC1; Annexin-1; Annexin A1; Lipocortin I; Annexin I; p35; Calpactin-2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-PAssay - dependent
WB1 - 3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16952 Mouse ANXA1

GeneID: 25380 Rat ANXA1

GeneID: 301 Human ANXA1

Background Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 39 kDa
PTM Phosphorylated by protein kinase C, EGFR and TRPM7 (PubMed:2457390, PubMed:15485879). Phosphorylated in response to EGF treatment (PubMed:2532504).
Sumoylated.