ARG58211

anti-Annexin A4 antibody

anti-Annexin A4 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Annexin A4
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Annexin A4
Antigen Species Human
Immunogen Synthetic peptide corresponding to a sequence in the middle region of Human Annexin IV (119-152aa EEIRRISQTYQQQYGRSLEDDIRSDTSFMFQRVL), different from the related Mouse and Rat sequences by three amino acids.
Conjugation Un-conjugated
Alternate Names PAP-II; 35-beta calcimedin; Protein II; Annexin IV; ANX4; PP4-X; P32.5; Annexin A4; Annexin-4; Carbohydrate-binding protein p33/p41; Lipocortin IV; HEL-S-274; Endonexin I; Placental anticoagulant protein II; PIG28; Chromobindin-4; ZAP36

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:1000
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11746 Mouse ANXA4

GeneID: 307 Human ANXA4

Swiss-port # P09525 Human Annexin A4

Swiss-port # P97429 Mouse Annexin A4

Gene Symbol ANXA4
Gene Full Name annexin A4
Background Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. [provided by RefSeq, Jul 2008]
Function Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. [UniProt]
Calculated MW 36 kDa