ARG55756

anti-Antithrombin III antibody

anti-Antithrombin III antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes Antithrombin III
Tested Reactivity Hu, Ms
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Antithrombin III
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 364-393 (C-terminus) of Human Antithrombin III.
Conjugation Un-conjugated
Alternate Names AT3D; Antithrombin-III; ATIII; Serpin C1; AT3; THPH7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
ICC/IF1:10 - 1:50
IHC-P1:50 - 1:100
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human blood plasma

Properties

Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide.
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11905 Mouse SERPINC1

GeneID: 462 Human SERPINC1

Swiss-port # P01008 Human Antithrombin-III

Swiss-port # P32261 Mouse Antithrombin-III

Gene Symbol SERPINC1
Gene Full Name serpin peptidase inhibitor, clade C (antithrombin), member 1
Background The protein encoded by this gene is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. More than 120 mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency. [provided by RefSeq, Jul 2009]
Function Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. [UniProt]
Cellular Localization Secreted, extracellular space.
Calculated MW 53 kDa
PTM Phosphorylated by FAM20C in the extracellular medium.