ARG10770

anti-Apolipoprotein AI antibody

anti-Apolipoprotein AI antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Apolipoprotein AI
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Apolipoprotein AI
Antigen Species Human
Immunogen Synthetic peptide around aa. 25-267 of Human Apolipoprotein A1.
Conjugation Un-conjugated
Alternate Names ApoA-I; ProapoA-I; Apo-AI; Apolipoprotein A-I; Apolipoprotein A1; 1-242

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P0.5 - 2 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Citrate buffer (10 mM, pH 6.0), Boil bathing for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 335 Human APOA1

Swiss-port # P02647 Human Apolipoprotein A-I

Gene Symbol APOA1
Gene Full Name apolipoprotein A-I
Background This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]
Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. [UniProt]
Calculated MW 31 kDa
PTM Glycosylated.
Palmitoylated.
Phosphorylation sites are present in the extracellular medium.