ARG64652

anti-Apolipoprotein B antibody

anti-Apolipoprotein B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Apolipoprotein B
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Apolipoprotein B
Antigen Species Human
Immunogen C-TDLHLRYQKDKK
Conjugation Un-conjugated
Alternate Names Apolipoprotein B-100; apoB-48; Apo B-100; apoB-100; Apo B-48; FLDB; LDLCQ4; ApoB

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 µg/ml
WB0.3 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 338 Human APOB

Swiss-port # P04114 Human Apolipoprotein B-100

Gene Symbol APOB
Gene Full Name apolipoprotein B
Background This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]
Function Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 516 kDa
PTM Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.