ARG22361
anti-Aquaporin 4 antibody
anti-Aquaporin 4 antibody for ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat
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Publication2
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Aquaporin 4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Aquaporin 4 |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide around the C-terminus of Rat Aquaporin 4. (N-CTKGSYMEVEDNRSQVETED) |
| Conjugation | Un-conjugated |
| Alternate Names | HMIWC2; Aquaporin-4; Mercurial-insensitive water channel; AQP-4; MIWC; WCH4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Aqp4 |
| Gene Full Name | aquaporin 4 |
| Background | This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. [UniProt] |
| Cellular Localization | Membrane |
| Calculated MW | 35 kDa (unmodified); 50 kDa (glycosylated) |
| PTM | Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40% (By similarity). |
Customer's Feedback
Average
Review for anti-Aquaporin 4 antibody
Application：WB
Sample：MCF7 and U87
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
AQP4 Attenuated TRAF6/NFκB Activation in Acrylamide-Induced Neurotoxicity
WB / Rat
Sevoflurane enhanced the clearance of Aβ1-40 in hippocampus under surgery via up-regulating AQP-4 expression in astrocyte.
WB, ICC/IF / Rat
