ARG64392

anti-Arylsulfatase B antibody

anti-Arylsulfatase B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Death antibody; Controls and Markers antibody; Metabolism antibody

Overview

Product Description Goat Polyclonal antibody recognizes Arylsulfatase B
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms (NP_000037.2; NP_942002.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Arylsulfatase B
Antigen Species Human
Immunogen C-KLARGHTNGTKPLD
Conjugation Un-conjugated
Alternate Names N-acetylgalactosamine-4-sulfatase; EC 3.1.6.12; MPS6; ASB; G4S; Arylsulfatase B

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 - 10 µg/ml
WB0.03 - 0.1 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 411 Human ARSB

Swiss-port # P15848 Human Arylsulfatase B

Background Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Area Cancer antibody; Cell Death antibody; Controls and Markers antibody; Metabolism antibody
Calculated MW 60 kDa
PTM The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. This post-translational modification is severely defective in multiple sulfatase deficiency (MSD).