ARG57174

anti-Aspartate Aminotransferase antibody [3G11]

anti-Aspartate Aminotransferase antibody [3G11] for Western blot,Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody [3G11] recognizes Aspartate Aminotransferase
Tested Reactivity Hu
Tested Application FACS, WB
Host Mouse
Clonality Monoclonal
Clone 3G11
Isotype IgG2a, kappa
Target Name Aspartate Aminotransferase
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-413 of Human Aspartate Aminotransferase
Conjugation Un-conjugated
Alternate Names Cysteine transaminase, cytoplasmic; cAspAT; GIG18; Glutamate oxaloacetate transaminase 1; cCAT; EC 2.6.1.3; Cysteine aminotransferase, cytoplasmic; ASTQTL1; AST1; EC 2.6.1.1; Transaminase A; Aspartate aminotransferase, cytoplasmic

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2805 Human GOT1

Swiss-port # P17174 Human Aspartate aminotransferase, cytoplasmic

Gene Symbol GOT1
Gene Full Name glutamic-oxaloacetic transaminase 1, soluble
Background Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]
Function Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. [UniProt]
Calculated MW 46 kDa