ARG63151
anti-Aurora A antibody
anti-Aurora A antibody for Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Aurora A |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IP, WB |
| Specificity | Reported variants represent identical protein: NP_003591.2; NP_940835.1; NP_940836.1; NP_940837.1; NP_940838.1; NP_940839.1 |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Aurora A |
| Antigen Species | Human |
| Immunogen | C-QNKESASKQS |
| Conjugation | Un-conjugated |
| Alternate Names | ARK-1; AIK; BTAK; Serine/threonine-protein kinase 6; Breast tumor-amplified kinase; Serine/threonine-protein kinase aurora-A; STK15; Serine/threonine-protein kinase 15; AURORA2; Aurora-related kinase 1; hARK1; AURA; STK6; STK7; Aurora kinase A; EC 2.7.11.1; Aurora/IPL1-related kinase 1; Aurora 2; ARK1; PPP1R47 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AURKA |
| Gene Full Name | aurora kinase A |
| Background | The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| Calculated MW | 46 kDa |
| PTM | Activated by phosphorylation at Thr-288; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-288 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-288 upon TPX2 binding. Thr-288 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-288 during mitosis. Phosphorylation at Ser-342 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-51 at the end of mitosis. Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome. Ubiquitinated by CHFR, leading to its degradation by the proteasome (By similarity). Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome. |
