ARG42744

anti-B7-H4 antibody

anti-B7-H4 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes B7-H4
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name B7-H4
Antigen Species Human
Immunogen Recombinant protein corresponding to T57-A258 of Human B7-H4.
Conjugation Un-conjugated
Alternate Names B7H4; B7 homolog 4; Protein B7S1; V-set domain-containing T-cell activation inhibitor 1; B7-H4; B7X; T-cell costimulatory molecule B7x; B7h.5; VCTN1; PRO1291; Immune costimulatory protein B7-H4; B7S1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 79679 Human VTCN1

Swiss-port # Q7Z7D3 Human V-set domain-containing T-cell activation inhibitor 1

Gene Symbol VTCN1
Gene Full Name V-set domain containing T cell activation inhibitor 1
Background This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Function Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. [UniProt]
Cellular Localization Cell membrane; Single-pass type I membrane protein. Note=Expressed at the cell surface. A soluble form has also been detected. [UniProt]
Calculated MW 31 kDa
PTM N-glycosylated. [UniProt]