ARG63391
anti-BAG3 antibody
anti-BAG3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
1
Overview
| Product Description | Goat Polyclonal antibody recognizes BAG3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | BAG3 |
| Antigen Species | Human |
| Immunogen | C-SSMTDTPGNPAAP |
| Conjugation | Un-conjugated |
| Alternate Names | BAG-3; BIS; CAIR-1; Bcl-2-binding protein Bis; BAG family molecular chaperone regulator 3; MFM6; Docking protein CAIR-1; Bcl-2-associated athanogene 3 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by antigen affinity chromatography. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O95817 Human BAG family molecular chaperone regulator 3 |
|---|---|
| Background | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody |
| Calculated MW | 62 kDa |
Customer's Feedback
Excellent
Review for anti-BAG3 antibody
Application：WB
Sample：293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
