ARG42703

anti-BDH1 antibody

anti-BDH1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes BDH1
Tested Reactivity Hu, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BDH1
Antigen Species Human
Immunogen Recombinant protein corresponding to M1-Y287 of Human BDH1.
Conjugation Un-conjugated
Alternate Names BDH; D-beta-hydroxybutyrate dehydrogenase, mitochondrial; Short chain dehydrogenase/reductase family 9C member 1; SDR9C1; EC 1.1.1.30; 3-hydroxybutyrate dehydrogenase

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 32 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.01% Sodium azide and 4% Trehalose.
Preservative 0.01% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 117099 Rat BDH1

GeneID: 622 Human BDH1

Swiss-port # P29147 Rat D-beta-hydroxybutyrate dehydrogenase, mitochondrial

Swiss-port # Q02338 Human D-beta-hydroxybutyrate dehydrogenase, mitochondrial

Gene Symbol BDH1
Gene Full Name 3-hydroxybutyrate dehydrogenase, type 1
Background This gene encodes a member of the short-chain dehydrogenase/reductase gene family. The encoded protein forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific requirement for phosphatidylcholine for optimal enzymatic activity. The encoded protein catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the two major ketone bodies produced during fatty acid catabolism. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]
Cellular Localization Mitochondrion inner membrane. Mitochondrion matrix. [UniProt]
Calculated MW 38 kDa