ARG58201

anti-BDNF antibody

anti-BDNF antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes BDNF
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BDNF
Antigen Species Human
Immunogen Partial recombinant protein corresponding to aa. 129-247 of Human BDNF.
Conjugation Un-conjugated
Alternate Names Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
IHC-P0.5 - 1 µg/ml
WB0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0) for 20 min followed by cooling at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 28 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12064 Mouse BDNF

GeneID: 24225 Rat BDNF

GeneID: 627 Human BDNF

Gene Symbol BDNF
Gene Full Name brain-derived neurotrophic factor
Background The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]
Function During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt]
Cellular Localization Cytoplasmic, secreted. [UniProt]
Highlight Related products:
BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins; Anti-Rabbit IgG secondary antibodies;
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Calculated MW 28 kDa
PTM The propeptide is N-glycosylated and glycosulfated.

Converted into mature BDNF by plasmin (PLG). [UniProt]