ARG65673

anti-BDNF antibody

anti-BDNF antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human,Mouse

Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
publication_link Publication3

Overview

Product Description Goat Polyclonal antibody recognizes BDNF
Tested Reactivity Hu, Ms
Predict Reactivity Dog, Rat
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Specificity This antibody is expected to recognise all reported isoforms (NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1)
Host Goat
Clonality Polyclonal
Target Name BDNF
Antigen Species Human
Immunogen Synthetic peptide around the internal region of Human BDNF (ETKCNPMGYTKE)
Conjugation Un-conjugated
Alternate Names Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF10 µg/ml
IHC-Fr1-3 µg/ml
IHC-PAssay - dependent
WBAssay dependent
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: WB was tested by the publication (dio: 10.21203/rs.3.rs-929317/v1)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12064 Mouse BDNF

GeneID: 627 Human BDNF

Swiss-port # P21237 Mouse Brain-derived neurotrophic factor

Swiss-port # P23560 Human Brain-derived neurotrophic factor

Gene Symbol BDNF
Gene Full Name brain-derived neurotrophic factor
Background The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]
Function During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt]
Highlight Related products:
BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins;
Related news:
The role of HDGF in tumor angiogenesis
Research Area Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
Calculated MW 28 kDa
PTM The propeptide is N-glycosylated and glycosulfated.
Converted into mature BDNF by plasmin (PLG).

Specific References

G721-0282 Exerts Anxiolytic-Like Effects on Chronic Unpredictable Mild Stress in Mice Through Inhibition of Chitinase-3-Like 1-Mediated Neuroinflammation

WB / Mouse

Hyeon Joo Ham et al.
Front Cell Neurosci,  (2022)

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Neuroprotective Effects of Estrogen Through BDNF-Transient Receptor Potential Channels 6 Signaling Pathway in the Hippocampus in a Rat Model of Perimenopausal Depression

function / 

Qiaoli Song et al.
Front Aging Neurosci.,  (2022)

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p53 Knock Out Mice Enhances Anxiety- and Depression-Like Behaviors through an Increase of Glutamate, Calcium, and Cytokine-Mediated Cell Death Signals

WB / Mouse

In Jun Yeo  et al.
Research Square,  (2021)

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